Use Of Reporter Genes With Introns

Use Of Reporter Genes With Introns

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Control of endogenous gene expression timing by introns ...

    https://genomebiology.biomedcentral.com/articles/10.1186/gb-2011-12-3-107#:~:text=Presence%20of%20introns%20in%20reporter%20genes%20alters%20timing,and%20intron-less%20%28In%20%28-%29%29%20reporter%20constructs%20%28Figure%201%29.
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Reporter gene fusions

    http://www.wormbook.org/chapters/www_reportergenefusions/reportergenefusions.html
    Translational reporters can provide a faithful representation of a gene's expression pattern because additional regulatory information that may be present in introns or 3 ′ UTRs is included in such reporter constructs. When mutants are available, translational reporters can be used in rescue experiments.

The Positive Effects of Introns on Plant Gene Expression ...

    https://portal.nifa.usda.gov/web/crisprojectpages/0207144-the-positive-effects-of-introns-on-plant-gene-expression.html
    A careful quantitative evaluation of reporter gene expression in each will be performed, with the aim of determining the distribution of enhancing sequences in an intron. In the second specific aim, a complementary approach will be used to identify potential enhancing sequences by computational means.

The Green Fluorescent Protein Gene Functions as a …

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC92671/
    The enhanced green fluorescent protein (GFP) gene (egfp) was used as a reporter of gene expression driven by the glyceraldehyde-p-dehydrogenase (gpd) gene promoter and the manganese peroxidase isozyme 1 (mnp1) gene promoter in Phanerochaete chrysosporium.Four different constructs were prepared. pUGGM3′ and pUGiGM3′ contain the P. chrysosporium gpd …

The Positive Effects of Introns on Plant Gene Expression ...

    https://portal.nifa.usda.gov/web/crisprojectpages/0195844-the-positive-effects-of-introns-on-plant-gene-expression.html
    The main approach used was to create a series of reporter genes, each with a single intron at one of eight different locations, and then to measure the ability of the intron to elevate expression from each position in single-copy transgenic Arabidopsis plants. This analysis revealed two distinct mechanisms through which introns affect expression.

Control of endogenous gene expression timing by introns ...

    https://genomebiology.biomedcentral.com/articles/10.1186/gb-2011-12-3-107
    Presence of introns in reporter genes alters timing of protein expression To test whether intron-related time delays could affect gene expression, Takashima et al. [ 7] generated intron-containing (In (+)) and intron-less (In (-)) reporter constructs (Figure 1 ).

Introns control stochastic allele expression bias | Nature ...

    https://www.nature.com/articles/s41467-021-26798-4
    Reporter alleles with introns expressed from chromosome V significantly reduced intrinsic noise (Fig. 2e, f and Supplementary Table 1, P = …

High-throughput construction of intron-containing hairpin ...

    https://pubmed.ncbi.nlm.nih.gov/22675447/
    With the wide use of double-stranded RNA interference (RNAi) for the analysis of gene function in plants, a high-throughput system for making hairpin RNA (hpRNA) constructs is in great demand. Here, we describe a novel restriction-ligation approach that provides a simple but efficient construction of intron-containing hpRNA (ihpRNA) vectors.

Intron enhancement of gene expression and the splicing ...

    https://link.springer.com/article/10.1007/BF00282645
    We have extended these studies with Adh1-S intron 1 using the firefly luciferase, Escherichia coli β-glucuronidase and chloramphenicol acetyltransferase reporter genes adjoined to different plant promoters and find enhancement of transient gene expression in all cases but one. We also show that the enhancement phenomenon can be mediated by the third intron of the maize actin gene.

Human intronless genes: Functional groups, associated ...

    https://www.sciencedirect.com/science/article/pii/S0006291X12011874
    Being intronless, cDNAs face even bigger problems with mRNA processing than naturally intronless mRNAs because, as recapitulated above, the latter possess specific adaptor-binding elements, enabling them to use one of the cellular pathways. Reporter studies examining intron effects on mammalian gene expression, although displaying great variability in the …

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