Lux Reporter Plasmid

Lux Reporter Plasmid

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Use of a lux reporter system for monitoring rapid changes ...

    https://academic.oup.com/femsle/article/188/1/29/534218
    To determine whether the luxA–luxB reporter system is suitable as a sensitive reporter for rapid real-time measurements of α-toxin gene (cpa) expression in Clostridium perfringens, and to widen the range of α-toxin-producing C. perfringens strains examined with respect to cpa expression during growth, the reporter plasmid pPS14 (possessing the α-toxin …

Improved lux reporters for use in Staphylococcus aureus

    https://pubmed.ncbi.nlm.nih.gov/19399993/
    The use of luxABCDE (lux) offers certain advantages over other reporters, such as: lacZ and xylE. It is real time and its signal generation is produced without the requirement for any additional substrates. In some bacteria such as Staphylococcus spp, light production by luciferase is restricted bec …

A stable luciferase reporter plasmid for in vivo imaging ...

    https://link.springer.com/article/10.1007/s00253-015-7229-2
    In the attempt to generate a stabilized lux-reporter plasmid that could be possibly used with different Gram-positive bacteria, we used the stabilization sequences from pIP501, a plasmid isolated from Enterococcus faecalis and Enterococcus faecium strains, which was reported as the conjugative plasmid with the broadest host range in Gram-positive bacteria, …

pMU1, an integrating lux reporter plasmid. tfd , the major ...

    https://www.researchgate.net/figure/pMU1-an-integrating-lux-reporter-plasmid-tfd-the-major-transcriptional-terminator-of_fig2_6470237
    Previously, to detect topA activity changes, we used a lux reporter plasmid (pFLUXptopA) in which the topA promoter controls the transcription of the luxCDABE reporter genes [64].

Toxin-antitoxin-stabilized reporter plasmids for ...

    https://pubmed.ncbi.nlm.nih.gov/24061415/
    Plasmid-based luciferase reporters could easily be introduced into a variety of GAS strains and the signals correlated linearly with viable cell counts. Co-expression of the streptococcal ω-ε-ζ toxin-antitoxin operon provided segregational stability in the absence of antibiotics for at least 17 passages in vitro and up to 7 days in a mouse infection model.

Different bacterial host-based lux reporter array for fast ...

    https://link.springer.com/article/10.1007/s00216-020-02943-8
    The lux genes were transformed in E. coli, B. subtilis, and A. tumefaciens by using the pBAV1K-T5-Lux plasmid [], and the corresponding bioluminescent recombinant bacteria were named as E. coli-lux (EC-lux), B. subtilis-lux (BS-lux), and A. tumefaciens-lux (AT-lux), respectively.The T5/lac promoter ensured the constitutive expression of the lux genes without …

Addgene: 8xGTIIC-luciferase

    https://www.addgene.org/34615/
    The plasmid was derived from the 4XGTIIC-lux made from Ian Farrance, by adding 4 more TEAD binding sites (ACATTCCA).

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