Luciferase Reporter Genes

Luciferase Reporter Genes

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Application of Firefly Luciferase (Luc) as a Reporter Gene ...

    https://pubmed.ncbi.nlm.nih.gov/32945904/
    Application of Firefly Luciferase (Luc) as a Reporter Gene for the Chemoautotrophic and Acidophilic Acidithiobacillus spp. Acidithiobacillus spp. are the most active bacteria in bioleaching and bioremediation, because of their remarkable extreme environmental adaptabilities and unique metabolic characteristics.

Construction of luciferase reporter gene vector for human ...

    https://pubmed.ncbi.nlm.nih.gov/20818070/
    The relative luciferase activities were detected in the transfected A549 cells. Results: Sequence analysis indicated that there were many cis-acting elements in the regions of 1 348 bp DNA sequence at the human MUC5AC gene 5 end. The 4 reporter gene vectors with promoter segments with different lengths were constructed successfully.

The Luciferase Reporter Assay: How it Works & Why You ...

    https://bitesizebio.com/10774/the-luciferase-reporter-assay-how-it-works/
    The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously.

Luciferase reporter gene assay on human 5-HT receptor ...

    https://pubmed.ncbi.nlm.nih.gov/25622827/
    The luciferase reporter gene assay, based on the responsible-element-regulated expression of luciferase, has been widely applied in the high-throughput functional assay for many targets because of its high sensitivity and reliability.

pGL4 Luciferase Reporter Vectors

    https://www.promega.com/-/media/files/resources/protocols/technical-manuals/0/pgl4-luciferase-reporter-vectors-protocol.pdf?rev=d1a2cdb9503847cf90a1a732b2212c57&sc_lang=en
    The pGL4 Luciferase Reporter Vectors(a–e) are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more

Reporter gene comparison demonstrates interference of ...

    https://www.nature.com/articles/s41598-020-80451-6
    These unstable reporter variants are employed for both fluorescent and luminescent reporter genes, such as destabilization domain-tdTomato (DD-tdTomato) and Nanoluc-PEST (NLucP) 15, 18, 19. The...

Bioluminescent Reporters | Reporter Gene Applications | An ...

    https://www.promega.com/resources/guides/cell-biology/bioluminescent-reporters/
    Diagram of firefly, Renilla and NanoLuc® luciferase chemical reactions with their respective …

Genetic Reporter Vectors | Reporter Cell Lines | Reporter Gene

    https://www.promega.com/products/luciferase-assays/genetic-reporter-vectors-and-cell-lines/
    Genetic Reporter Vectors and Cell Lines. We offer luciferase reporter vectors containing NanoLuc®, firefly, and Renilla reporter genes. These include promoterless vectors for cloning your regulatory region of interest, constructs encoding short half-life reporter genes for a more immediate response to changes in transcription levels, and vectors for creating protein …

Bioluminescent Reporter Genes - Promega

    https://www.promega.com/resources/pubhub/enotes/bioluminescent-reporter-genes/
    To provide reporters designed to meet different experimental needs, families of luciferase genes have been developed yielding different intracellular stabilities. Promega has developed destabilized luciferase reporters by genetically fusing a protein degradation sequence to the luciferase genes. Due to their increased rate of degradation, these destabilized reporters …

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