Luciferase Reporter Construct

Luciferase Reporter Construct

The links below contain various information about Luciferase Reporter Construct that might interest you. The links below contain various information about the keyword that might interest you. Review information from various sources to form a position based on as much information as possible.


Construction of luciferase reporter gene vector for human ...

    https://pubmed.ncbi.nlm.nih.gov/20818070/
    Objective: To clone the human mucin (MUC) 5AC gene promoter and construct its luciferase reporter vector for human MUC5AC gene and analyze its transcriptional activity. Methods: The 1 348 bp DNA sequence at the human MUC5AC gene 5 end was analyzed by the Vector NTI software. After the target sequence from human A549 cells genomic DNA was amplified by …

[Construction and identification of luciferase reporter ...

    https://pubmed.ncbi.nlm.nih.gov/25985524/
    Abstract. The aim of this study is to clone the mouse T-bet promoter and enhancer, construct and identify the firefly luciferase reporter gene plasmid pGL4.10-TBX21pr-CNS for T-bet transcription regulation study and its function in signaling of multiple sclerosis. The promoter and CNS of T-bet were predicted by bioinformatics assay.

Luciferase-based reporter to monitor the transcriptional ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748892/
    Luciferase reporter results after transfection of wildtype sequence or mutated estrogen-related receptor α (ERRα) reporter constructs plus vector or ERRα-HA overexpression constructs in human 293T cells.

The Luciferase Reporter Assay: How it Works & Why You ...

    https://bitesizebio.com/10774/the-luciferase-reporter-assay-how-it-works/
    The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously.

Luciferase - Wikipedia

    https://en.wikipedia.org/wiki/Luciferase
    In biological research, luciferase is commonly used as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Additionally, proluminescent molecules that are converted to luciferin upon activity of a particular enzyme ...

Luciferase reporters—Ready-To-Glow secreted luciferase assay

    https://www.takarabio.com/products/cell-biology-assays/reporter-systems/secreted-luciferase-assay
    13 rows

Noninvasive Bioluminescence Imaging in Small Animals

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614121/
    Plasmid or viral techniques are effective for the transfection of cancer cells with the luciferase reporter construct. Figure 1 shows an image of a firefly luciferase–positive xenografted tumor in the mammary fat pad of a mouse. The human breast cancer cells (2LMP, a subclone of MDA-MB-231) were stably expressing the luciferase gene under control of the CMV promoter, using an …

PDLIM1 inhibits NF-κB-mediated inflammatory signaling by ...

    https://www.nature.com/articles/srep18327/
    (a) Luciferase activity in MEF transfected with a pGL4-NF-κB luciferase reporter construct along with or without a plasmid encoding PDLIM1, then left untreated or treated with LPS (2 and 10 ng/ml ...

SKM Haryana announces to continue protest

    https://www.dailypioneer.com/2021/state-editions/skm-haryana-announces-to-continue-protest.html
    SKM Haryana announces to continue protest. Samyukt Kisan Morcha (SKM), Haryana unit on Wednesday announced that the farmers from the State will not return to their homes from the agitation site at ...

Luciferase reporter assays: Powerful, adaptable tools for ...

    https://www.promega.com/~/media/files/resources/cell%20notes/cn021/luciferase%20reporter%20assays-%20powerful%20adaptable%20tools%20for%20cell%20biology%20research.pdf?la=en
    assaying for the luciferase reporter gene. The studies showed that cells transfected with the variant –764G construct had a two- to threefold higher luciferase activity than the common –764C construct, indicating that the G allele conferred a higher promoter activity. The fact that the G …

We are sure that you have received all the information you are interested in about Luciferase Reporter Construct. We recommend that you also look at data on similar issues related to journalism.

Related Pages

New Pages

Previous Page | Next Page